Abstract: This paper analyzes the development trend and hot spots of bioinformatics in the world,provides literature support for researchers in the field, and offers suggestions and guidance for the development of bioinformatics in China in the future. Based on the 11 international bioinformatics journals published from 1998 to 2019 by SCIE, the authors adopt the bibliometrics method to describe the issuing countries and institutions. Histcite and Pajek are used to analyze the literature from 1998 to 2014, whereas the main path is elaborated through visual analysis. BICOMB and gcluto are then used to conduct keyword clustering analysis on literature from 2015 to 2019 to explore research hotspots. According to the literature information in the citation chronology chart and the main path chart, the research hotspots in 9 aspects of bioinformatics from 1998 to 2014 are summarized and 3 main paths are selected. 6 research hotspots in the field of bioinformatics from 2015 to 2019 are obtained.